Simultaneous determination of multi-class veterinary drugs in fishery products with liquid chromatography–tandem mass spectrometry

Abstract The objective of this study was to optimize the analytical method for multi-class veterinary drug residues 64 compounds in fishery products. Several from drugs are banned or unauthorized products according Korean Food Code. Samples were extracted using acetonitrile/water (4:1, v/v) and clean-up step carried out by adding octadecylsilane acetonitrile-saturated hexane. target confirmed quantified liquid chromatography–tandem mass spectrometry (LC–MS/MS). proposed validated CODEX guidelines (CAC/GL-71), most found be acceptable quantities under requirements validation guidelines. recovery analytes typically 60–120% range, precision, expressed as coefficient variation less than 31% at all levels concentration. limit quantification ranged 0.03 3 μg kg −1 Moreover, application 96 real samples demonstrated that no exceeded maximum residue limits (MRLs). This evaluation provides reliable identification can an efficient means inspect currently not approved aquaculture Korea.